Translation Initiation: Extract Systems and Molecular Genetics

Translation Initiation: Extract Systems and Molecular Genetics

4.11 - 1251 ratings - Source

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.5.4. Immunoprecipitation reaction and detection of precipitated proteins 1. Add 200 mg WCE (as estimated by the Bradford assay) to ... portion (usually 10 ml or 5%) by immunoblotting. 4. Wash the resin three times with 200 ml of Buffer A containing 0.1% of Triton X-100. ... problem with this assay occurs when the epitope is sterically sequestered. ... REFERENCES Asano, K., and Hinnebusch, A. G. (2001).

Title:Translation Initiation: Extract Systems and Molecular Genetics
Publisher:Academic Press - 2007-10-15

You must register with us as either a Registered User before you can Download this Book. You'll be greeted by a simple sign-up page.

Once you have finished the sign-up process, you will be redirected to your download Book page.

How it works:
  • 1. Register a free 1 month Trial Account.
  • 2. Download as many books as you like (Personal use)
  • 3. Cancel the membership at any time if not satisfied.

Click button below to register and download Ebook
Privacy Policy | Contact | DMCA